Plasmid

Part:BBa_K3978046:Design

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


reget-plasmid-CLPX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 1313
    Illegal PstI site found at 1414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 1395
    Illegal PstI site found at 1414
    Illegal NotI site found at 1432
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 1395
    Illegal BglII site found at 879
    Illegal BglII site found at 1588
    Illegal BamHI site found at 1389
    Illegal XhoI site found at 1637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 1313
    Illegal PstI site found at 1414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 1313
    Illegal PstI site found at 1414
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 1954
    Illegal NgoMIV site found at 6631
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2539
    Illegal SapI.rc site found at 4199


Design Notes

The promoter should not be too stronger.


Source

This part is designed by ourselves.

References